Substitute George Lapslie rescued a point for Gillingham at home to Cheltenham in an entertaining 2-2 draw.
Robbie McKenzie was the match-winner as Gillingham ended MK Dons' unbeaten run with a 1-0 win at Stadium MK.
Jayden Clarke's second-half strike was enough to give Gillingham all three points with a 1-0 win against Salford at Priestfield.
Jude Arthurs’ second-half goal enabled Bromley to beat Gillingham 2-1 and record their fourth win of the Sky Bet League Two season.
Harrogate came from behind at Priestfield to secure back-to-back Sky Bet League Two wins with a 2-1 victory.
Who are the top team in the land? Which clubs are punching above their weight and which historic powerhouses languish in the lower divisions?
Jayden Clarke came off the bench to score the winning goal as Gillingham ended their run of seven games without a win by stunning League Two leaders Port Vale 1-0.
Follow updates from all of Saturday's matches in the Sky Bet Championship, League One and League Two here.
New Swindon boss Ian Holloway saw his side snatch a last-gasp 1-1 draw with Gillingham despite playing most of the match with 10 men.
First-half goals from Matt Baker and Kyle Hudlin secured Newport a much-needed 2-0 win at out-of-sorts Gillingham.
Glenn Morris tells Sky Sports how he is still pulling off stunning saves in the EFL at the age of 40 in goal for Gillingham.
Bradford registered a third successive League Two win as they came from behind to beat Gillingham 2-1.
Gillingham's Mark Bonner on leaving Cambridge, the benefits of his early appointment at Priestfield, Bradley Dack's return, a realistic outlook on the season and more.
Shilow Tracey netted an impressive double as Crewe beat Gillingham 2-0.
Free highlights from all of Saturday's matches in the Championship.
Jayden Clarke came off the bench to score a second-half brace as 10-man Gillingham took all three points at Priestfield to end Tranmere's unbeaten start to the season.
Follow Saturday's Sky Bet EFL action here...
Saturday's Sky Bet Championship, League One and League Two matches here...