Napoli's dream of winning the Serie A title lies in tatters after they were humbled 3-0 by a Giovanni Simeone-inspired Fiorentina.
Benevento were relegated to Serie A the day after celebrating their shock victory at AC Milan as results elsewhere on Sunday condemned them to the drop.
Inter Milan moved up to third place in Serie A with a comfortable 4-0 victory over Cagliari at San Siro.
Juventus bounced back from their European exit by extending their lead atop Serie A to six points.
Cagliari eased their Serie A relegation fears courtesy of Luca Ceppitelli's late winner against free-falling Udinese.
Amadou Diawara left it late to fire Napoli to a nervy 2-1 victory over Chievo that keeps their Serie A title hopes alive.
Atalanta saw their hopes of a top-six finish dented after a 2-1 defeat at home by Sampdoria, while rivals Fiorentina won 2-0 at Udinese.
Juventus warmed up for their Champions League clash with Real Madrid with a 3-1 victory over AC Milan.
AC Milan needed a late Andre Silva goal to snatch a 3-2 win at home to lowly Chievo.
Fiorentina and Cagliari have retired the No 13 shirt formerly worn by captain Davide Astori following the 31-year-old's death.
Leaders Napoli sent their Serie A winning streak into double figures with an emphatic 5-0 success at Cagliari.
Yann Karamoh's first goal for Inter Milan settled their fraught 2-1 victory over nine-man Bologna at the San Siro on Sunday.
Gonzalo Higuain scored a hat-trick as Juventus thrashed Sassuolo 7-0 to keep the pressure on Napoli in the Serie A title battle.
Dries Mertens scored twice as Napoli beat Bologna 3-1 to reclaim top spot in the Serie A table from reigning champions Juventus.
Napoli moved four points clear at the Serie A summit on Sunday with a 1-0 victory over Atalanta in Bergamo.
Fernando Bernardeschi scored the only goal as Juventus kept Serie A leaders Napoli in their sights with a 1-0 win over Cagliari.
Roma's bid to break into Serie A's top three was thwarted in part by two video assistant referee decisions as they drew 1-1 against Sassuolo.
Napoli took full advantage of Inter Milan's first defeat of the Serie A season to take over at the top.
Inter Milan remain in top spot in Serie A after holding Juventus to a goalless draw in Turin.
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