Joe Marchant and Paul Hill will miss England's tour to Argentina in June because of injury.
Marchant, centre for the Harlequins, arrived at the Lensbury-based fitness camp two weeks ago with soft-tissue irritation of his big toe but the problem persists despite treatment.
Hill, who has an ongoing neck injury, has not recovered in time and the prop will remain with Northampton Saints for his rehabilitation.
Denny Solomona will stay with Sale Sharks this week to continue treatment after suffering a mid-foot sprain in Brighton last week.
The winger will be assessed at the end of the week to decide if he will rejoin the England camp and continue his rehabilitation.
Eddie Jones will announce his 31-man squad travelling to Argentina on Monday following the weekend's Premiership Final and England's Old Mutual Wealth Cup match against the Barbarians.
Argentina tour squad vice-captains Mike Brown, Danny Care and George Ford are all included in the squad and head Jones is expected to hand one of that trio the armband.